Journal: Oncogene

Article Title: Peroxisome proliferator-activated receptor gamma is frequently downregulated in a diversity of sporadic nonmedullary thyroid carcinomas.

doi: 10.1038/sj.onc.1206400

Figure Lengend Snippet: Figure 2 Immunohistochemistry with anti-PPARg antibody. Except for four frozen FTC tumor sections, all tissues were paraffin embedded and most were analysed as components of tissue microarrays. Normal thyroid sections were included in each experiment as positive controls. Following dewaxing with xylene and antigen retrieval in citric acid buffer for 30 min at 941C, the PPARg (E8) mouse monoclonal antibody (sc-7273, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) was incubated at a dilution of one in 12 for 1 h at 371C. Detection was by the LSAB2 streptavidin-horseradish peroxidase system (DAKO Corp., Car- pinteria, CA, USA) and counterstaining with hematoxylin l. Slides were scored for the presence or absence of nuclear staining and the intensity of staining was compared to the normal control from the same experiment. (a) normal thyroid shows moderate staining in the nucleus; (b) FTC with complete absence of staining; and (c) translocation-positive FTC shows very strong nuclear staining

Article Snippet: A total of 142 sections were analysed, as detailed in Table 1b, including 17 of the 19 FTCs used for RT–PCR analysis, additional independent cases accrued through the Department of Pathology, The Ohio State University and a commercial human thyroid cancer tissue array (Imgenex IMH-319).

Techniques: Immunohistochemistry, Incubation, Staining, Control, Translocation Assay

Journal: Oncotarget

Article Title: MicroRNA-224 is implicated in lung cancer pathogenesis through targeting caspase-3 and caspase-7.

doi: 10.18632/oncotarget.5224

Figure Lengend Snippet: Figure 2: The expressions of CASP3 and CASP7 mRNA are down regulated in NSCLC. a. CASP7 mRNA expressions in thirty paired NSCLC tissues and NATs. b. CASP3 mRNA expressions in thirty paired NSCLC tissues and NATs. GAPAH was used for normalization. c. and d. Kaplan-Meier plots of overall survival of lung cancer patients, stratified by expression of CASP7 (1926 patients) c. or CASP3 (1926 patients) d.. Data obtained from the Kaplan-Meier plotter database (kmplot.com/analysis).

Article Snippet: nsclc tissue samples Human Lung Tissue Microarrays (IMH-358) were purchased from Novus Biologicals, San Diego, CA.

Techniques: Expressing

Journal: Oncotarget

Article Title: MicroRNA-224 is implicated in lung cancer pathogenesis through targeting caspase-3 and caspase-7.

doi: 10.18632/oncotarget.5224

Figure Lengend Snippet: Figure 4: Expressions of CASP3 and CASP7 are inversely correlated with miR-224. a. Representative pictures and summary of co-expression analyses for miR-224 and CASP7 in NSCLC tissues. MiR-224 was detected by using 5’-DIG labeled LNA probe (purple) and CASP7 was detected by immunohistochemistry (red). Left: High CASP7 and low/neg miR-224, Middle: similar expression of CASP7 and miR-224, right: Low/neg CASP7 and high miR-224. b. Representative pictures and summary of co-expression analyses for miR- 224 and CASP3 in NSCLC tissues. MiR-224 was detected by using 5’-DIG labeled LNA probe (purple) and CASP3 was detected by immunohistochemistry (red). Left: High CASP3 and low/neg miR-224, Middle: similar expression of CASP3 and miR-224, right: Low/ neg CASP3 and high miR-224. c. In the cases of NSCLC where both miR-224 and CASP7 expression were noted, no detectable CASP7 was found in cancer cells overexpressing miR-224 (purple arrow).

Article Snippet: nsclc tissue samples Human Lung Tissue Microarrays (IMH-358) were purchased from Novus Biologicals, San Diego, CA.

Techniques: Expressing, Labeling, Immunohistochemistry